The creatine kinase system and pleiotropic effects of creatine
نویسندگان
چکیده
منابع مشابه
NOVEL REAGENTS FOR CREATINE KINASE
The purpose of this investigation was to develop a simple colorimetric method for creatine kinase (CK). The new method is based on the reaction of creatine, formed enzymatically from creatine phosphate and ADP, with different glyoxal compounds. Hydrated glyoxals, such as para-nitrophenyl, 2-thiophene, 4- biphenyl, 4, 4' -biphenyl,α-naphthyl, β-naphthyl, para-chlorophenyl, and styryl were...
متن کاملCreatine and creatine kinase measurement.
*Many methods for measuring creatine are known, the principal ones being direct calorimetric assay (l), indirect calorimetric assay (2), and destruction with bacterial adaptive enzymes (3). However, none of these methods are specific nor are they sensitive enough to detect very small amounts of creatine. rIhese assays have been used to examine urine and serum for creatine content, but, because ...
متن کاملAn interlaboratory study of creatine kinase and creatine kinase isoenzymes.
Over 400 laboratories participated in an interlaboratory study of creatine kinase (EC 2.7.3.2) in which they were requested to analyze five lyophilized samples for total creatine kinase and creatine kinase isoenzymes and to complete a questionnaire regarding methodology. The specimens were prepared from an homogenate of human myocardium that had been centrifuged, dialyzed, and added to a matrix...
متن کاملCreatine-creatine phosphate shuttle modeled as two-compartment system at different levels of creatine kinase activity.
In order to characterize ADP-ATP and creatine-creatine phosphate (Cr-CrP) shuttles a minimal mathematical model with two compartments and cyclic turnover of matter was designed. The 'mitochondrial' compartment contained 'ATP-synthase' and 'mitochondrial creatine kinase' (mitCK). The 'cytoplasmic' compartment consisted of 'ATPase', 'cytoplasmic creatine kinase' (cytCK) and an 'ADP-binding struct...
متن کاملCreatine Kinase Activity and Cartilage Energy Metabolism: Creatine Kinase-creatine Phosphate Circuit Activity and Macromolecule Synthesis
Earlier studies showed that there is significant creatine kinase activity in the epiphyseal growth cartilage, and there is stage-specific isoform expression. To further explore the role of this enzyme in cartilage development, we disrupted the creatine kinase creatine phosphate (CK-CP) circuit using the creatine analogue, cyclocreatine. We found that while cyclocreatine decreased creatine kinas...
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ژورنال
عنوان ژورنال: Amino Acids
سال: 2011
ISSN: 0939-4451,1438-2199
DOI: 10.1007/s00726-011-0877-3